Suitable quantity and quality of freshwater are required for all living things. In the developing world, various countries are facing water crisis due to Industrialization, agricultural run-off, deforestation urbanization, increasing population, climatic changes, and other anthropogenic activity present in the aquatic ecosystems [1]. The concern for water pollution of surface waters is based on rivers and small lakes [2]. The surface water of Lakes is usually a vulnerable drinking water source because the quality of the lake water may depend upon the activities present in the watershed and it may affect the productivity and natural conditions of lakes [3]. Water pollution has negative effect on the living things present in the wetlands as well as on the environment [4].

Wetlands contain a rich and huge amount of biodiversity and it is also a most productive ecosystem which carries out critical regulatory functions of hydrological process within the watersheds [5]. Wetlands are known as “biological supermarkets” because of their excessive food chain and rich biodiversity, supporting millions of people [6]. Wetlands provide essential natural habitat to multiple wildlife such as amphibians, birds, fish species, invertebrates, plant species (macrophytes & plankton diversity), mammals, and as well as focal diversity to many others [7]. Due to the global gene pool, geographical location, dominant species, water chemistry soil or sediment characteristics, and nutrient limitation wetlands express rich diversity [8]. Plants that are bound to the water to complete their life cycle are known as aquatic plants, from that group, some plants are completely submerged and some are more or less growing along the water’s edge [9]. Macrophytes are a large group that possesses macroalgae (large size algae), mosses, ferns, and angiosperms (Flowering plants) [10]. Macrophytes play a key role in the aquatic ecosystem because they act as preliminary producer sources in the food chain of the aquatic ecosystem and maintain the proper equilibrium between abiotic and biotic components in the aquatic ecosystem [11]. Macrophytes are measurable indicators of the ecological conditions of surface waters [12]. However, the macrophyte properties are a beneficial bio-indicator of water quality are not involved only in its sensitivity to pollution, but also its selectivity. Remarkably, various macrophyte species strongly depend on the various Physicochemical parameters of water which has proved to be vulnerable to change in the aquatic ecosystem [13]. In the freshwater ecosystem, the presence of water pollutants adversely affects the structure and function of biotic components, aesthetic value, and environmental quality of the freshwater ecosystem [14]. Macrophyte species diversity is controlled by various abiotic components [15]. Many studies were conducted to understand the effect of industrial and domestic wastewater on the diversity of macrophytes [16,17]. The important aim of this investigation is to identify the difference in the native flora dominating in the polluted lake and non-polluted lake, in order to assess the pollution - macrophyte relationship and to evaluate the role of various polluting factors on the distribution and composition of the macrophyte flora in the selected lakes.

Diversity Indices: The Shannon-Weiner Index (H') reaches maximum levels (e.g., H'=2.55) in non-polluted, pristine environments, whereas it significantly degrades in polluted zones.

Species Dominance: The Simpson Dominance Index (D) inversely reflects pollution. While unpolluted zones support diverse, coexisting species, stressed environments (such as heavily loaded wastewater sites) show elevated dominance scores (e.g., D=0.19) by pollution-tolerant species like Lemna perpusilla or Eichhornia crassipes.

Photosynthetic Pigment Decline: Pollution limits plant productivity. Plants exposed to industrial effluents often face a reduction in chlorophyll levels of 15% to 50% compared to those in clean sites, alongside concomitant leaf yellowing and structural stunting.

Community Shift (Similarity Index): Contaminated sites display a Similarity Index of up to 88% compared to one another, reflecting the uniform, degraded flora dominated by marginal, non-indigenous species (e.g., Polygonum punctatum instead of indigenous flora).

Biological Indices: The River Macrophyte Hydraulic Index (RMHI) acts as a biological indicator of organic pollution; its values soar in downstream stations choked with pollutants and lacking swift-water velocities.

Total 45 vascular macrophytes were recorded, which belong to 26 families (Table:1), where Araceae was the most abundant family (6 species, 23.07 %) of the total flora, followed by the Cyperaceae family (5 species, 19.23 %), Boraginaceae (4 species, 15.38 %). Meanwhile, the following families consists two species (7.69 %) for each, Asteraceae, Commelinaceae, Convolvulaceae, Hydrocharitaceae, Lythraceae, Nymphaeceae, Poaceae, and another 16 families were considered as monospecific ones (Fig: 2). During the study, 31 species were in the polluted wetland (68.89 %) and 40 species were in the non-polluted wetland (88.89 %) and 26 species (57.78 %) were in both groups. From the results, it can be observed that the number of macrophyte species adapted to the highly polluted wetland is generally lower than the wetland which carried fewer pollutants. Group X (polluted wetland) presented 3.277 and group Y (non-polluted wetland) presented 3.574 Shannon’s index. A bivariate linear model separates the two groups. This application revealed a highly significant difference between the floristic composition of these two groups (Fig: 4). Remarkably Ammannia baccifera, Ipomoea fistulosa, and Leersia hexandra equally cover both groups . Eichhornia crassipes was the dominant species in the polluted wetland and Ceratophyllum demersum was the dominant at in the non – polluted wetland (Table: 1). On the other hand, many species ( Heliotropium indicum, Heliotropium ovalifolium, and Heliotropium supinum) of the Boraginaceae family represented only polluted wetland and was restricted to the non-polluted wetland. Similarly, Bacopa monnieri, Chara, Coldenia procumbens, Cyperus difformis, Grangea maderaspatana, Hydrolea zeylanica, Lemna trisulca, Limnophyton obtusifolium, Marselia quadrifolia, Nelumbo nucifera, Nymphaea nouchali, Nymphoides indicum, Pistia stratiotes, and Utricularia aurea were only non-polluted wetland and was found in polluted wetland (Table: 1). Other species found were scattered throughout the study period in small strands.

Table 1: Recorded Species, Families, Presence Percentage Frequency in Polluted Sites (F % In Pol.), Presence Percentage Frequency in Non-Polluted Sites (F% In Non.), Life Form Classification (LC) & Ecological Classification (EC) of the Recorded Species in the Studied Wetland

Note: EC (Ecological classification) abbreviations: FF= Free-floating, FR= Floating but rooted, SR= Submerged but rooted, SNR= Submerged but not rooted, AR= Amphibious and rooted, ER= Emergent but rooted. LC (Life form classification) abbreviations: CP= Chamophytes, HCP= Hemicryptophytes, CRP= Cryptophytes, TP= Therophytes.

(B): Percentage Composition of Life Form Classification

The relationship between the macrophytes and water variables was studied using Canonical correspondence analysis (CCA). Figure: 5 showed the significant correlation between the water parameters and Table: 2 showed inter-set correlations which is derived from the canonical correspondence analysis (CCA) of the examined water parameters. Heavy metal contamination was present in the polluted wetland but in the non-polluted wetland, the heavy metals were not in the detection range. Turbidity and Temperature showed a positive relationship. Due to the lake water sample, sites showed the basic pH which is highly positive with turbidity [25]. High DO (Dissolved Oxygen) indicates the less amount of contamination and high BOD (Biological Oxygen Demand) indicate a large amount of contamination present in the lake, DO and BOD were negatively correlated with each other (Fig: 5)

Table 2: Mean Values ± SE of the Water Variables in the Polluted and Non-Polluted Vegetation Groups (X And Y) of Macrophytes

Species Data: Macrophyte composition and abundance (phytosociology) across multiple sampling sites (e.g., presence-absence or percentage cover).

Environmental Data: Measured physicochemical parameters of the water and sediment at those sites (e.g., pH, Electrical Conductivity, Biological Oxygen Demand (BOD), Dissolved Oxygen (DO), and heavy metal concentrations). Ecological classification of lakes based on macrophyte phytosociology identifies non-polluted systems via high diversity, while polluted, eutrophic lakes are marked by low diversity, high density, and dominance of species like Eichhornia crassipes. Key quantitative metrics for polluted conditions include Shannon-Wiener index values below 1.5, Simpson dominance indices (D) above 0.6, and Abundance/Frequency (A/F) ratios exceeding 0.05.

Submerged vs. Emergent: Submerged macrophytes absorb metals directly through their thin cuticles across the entire water column. Emergent and floating plants (like Eichhornia crassipes or Typha angustata) rely primarily on root systems to filter sediment and extract metal ions [26].

Bioaccumulation: Macrophytes have a low capacity to regulate the uptake of heavy metals. They act as "chemical bioconcentrators" absorbing high concentrations without degrading them.

Pollution was there at the selected study site due to the high anthropogenic activities surrounding the study area. A general reduction of macrophytes present at group X is because of the high pollutants present in it as compared to group Y. Reduction in macrophyte diversity and species richness observed at studied wetland was supposedly linked to the pollution status of the water parameters which could exhibit phytotoxic effects on macrophytes and colonization process.

Species Loss & Dominance: As water quality degrades, sensitive submerged species (e.g., Hydrilla spp.) decline, while highly adaptive, free-floating plants (like Eichhornia crassipes / Water Hyacinth) thrive and create dense monocultures.

Reduced Diversity: Phytosociological indices (such as Species Richness, Frequency, and Dominance) notably decline in polluted reaches, showing an absence of rare or pollution-intolerant plant communities.

Growth Retardation: High concentrations of heavy metals (like Copper or Cadmium) inflict physiological stress, causing reduced growth rates, impaired reproduction, and damaged root cell membranes.

Nutrient Overload: Excess nitrogen and phosphorus (from agricultural runoff and urban sewage) stimulate explosive algal blooms.

Light & Nutrient Deprivation: This excessive algal and phytoplankton growth blankets the water surface and competes with rooted macrophytes for light, CO₂, and essential nutrients, ultimately stifling submerged plant growth.

The authors are thankful to SICART (Sophisticated Instrumentation Center for Applied Research and Testing), Vallabh Vidyanagar, Gujarat for analysis, and also thankful to UGC New Delhi – NFOBC for financial support.