Analytical Method Development and Validation are critical components of quality assurance in pharmaceutical, chemical, and biotechnological industries. Method development involves designing reliable procedures to accurately detect, identify, and quantify substances within a sample. It requires selecting appropriate techniques, such as chromatography or spectroscopy, and optimizing parameters to achieve the desired sensitivity, specificity, and reproducibility. Once developed, the method must be validated to confirm its accuracy, precision, linearity, robustness, and limits of detection and quantitation. Validation ensures the method meets regulatory standards and consistently produces trustworthy results. These processes are essential for drug formulation, stability testing, and regulatory approval. With growing demands for precision and compliance, analytical method development and validation remain foundational to product safety, efficacy, and overall scientific integrity.