Chromatography and Mass Spectrometry are highly advanced techniques used for the separation, identification, and analysis of chemical compounds. Chromatography works by separating components in a mixture based on their physical or chemical properties, making it ideal for analyzing complex substances. Mass Spectrometry complements this by identifying and quantifying compounds through their mass-to-charge ratio. When combined, these techniques offer unmatched sensitivity and accuracy, making them essential tools in fields like pharmaceuticals, environmental science, food safety, biotechnology, and forensic science. This powerful combination helps detect trace levels of substances, ensuring high-quality results in research and industry. With ongoing technological progress, Chromatography and Mass Spectrometry continue to evolve, providing deeper insights into molecular structures and advancing modern analytical applications globally.